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oriole fluorescent protein stain  (Bio-Rad)


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    Bio-Rad oriole fluorescent protein stain
    Oriole Fluorescent Protein Stain, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 95/100, based on 306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluorescent+protein/Oriole+Fluorescent+Gel+Stain/pmc13148924-196-35-39
    Average 95 stars, based on 306 article reviews
    oriole fluorescent protein stain - by Bioz Stars, 2026-09
    95/100 stars

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    Incubation:

    Article Title: Neonatal Microglia and Their Secretome as Mediators of Brain Repair
    Article Snippet: Thereafter, to block unspecific binding, sections were incubated in a blocking buffer (TSA kit, Invitrogen) for 90 min at RT. .. Next, sections were incubated with corresponding primary antibodies: rabbit-polyclonal to green fluorescent protein (GFP, with specificity against RFP) (1:200, Abcam, Cat. No. ab6556) and rat-anti-mouse MAC1 (1:200, Bio-Rad, Cat. No. MCA711) in 3% BSA in 0,2% PBST for 2h at RT. .. Following incubation, sections were rinsed with PBS and incubated with secondary antibodies: donkey-anti-rat Alexa Fluor 488 (1:200, Invitrogen, Cat. No. A21208) and poly-HRP-conjugated goat anti-rabbit secondary antibody (1:200, TSA kit, Invitrogen) for 1h at RT and overnight at 4°C.



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